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Cardiac Markers ELISA Kits

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Hong Kong

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Cardiac Markers ELISA Kits

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ELISA Tests

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U&U Healthcare International Limited

Hong Kong

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Contact Person Ms. Tracy

KLN, HK, HK

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Product Description

Cardiac Markers ELISA kits

CK-MB ELISA kit
Human CK-MB ELISA kit
CRP ELISA kit
Human C-Reactive Protein (CRP) ELISA Test Kit
Digoxin ELISA kit
Human Digoxin ELISA Test Kit
Lp(a) ELISA kit
Human Lipoprotein A ELISA Kit
Myoglobin ELISA Test
Cardiac marker human Myoglobin ELISA test kit
Troponin I ELISA Test
Cardiac marker human Troponin I ELISA test kit
CK-MB ELISA kit

Name	CK-MB ELISA Test
Full name	Human CK-MB ELISA kit
Category Name	Cardiac Markers ELISA kits
Test	96
Method	Enzyme Linked Immunosorbent
Principle	Peroxidase Conjugated Competitive ELISA
Detection Range	0-400ng/ml
Sample	25 ul serum
Specificity	96%
Sensitivity	0.5 ng/mL
Total Time	~45 min
Shelf Life	18 months from the manufacturing date

CK-MB ELISA kit description:

CK-MB ELISA kit is an Enzyme Linked Immunosorbent assay for the quantitative determination of Circulating Creatinine Kinase (MB-Isoform) concentrations in human serum.

Materials provided with CK-MB ELISA Test Kits:
1. Microplate: wells coated with Streptavidin
2. CK-MB EIA Kit Calibrators
3. CK-MB EIA Kit Enzyme Reagent
4. CK-MB EIA Test Substrate A: TMB solution
5. CK-MB EIA Test Substrate B: H2O2 solution
6. CK-MB ELISA Test Wash Solution
7. CK-MB ELISA Test Stop Solution

Materials & Instrumentations required, not included
1. Precision Pipettes
2. Distilled or deionized water
3. Quality Control Materials
4. ELISA kit Microplate Washer
5. ELISA kit Microplate Reader at 450 & 620nm wavelength

CK-MB ELISA kit Background Information:
Creatinine kinase (CK) is an enzyme, found primarily in muscle and brain tissue, which exists as three dimeric isoenzymes of CKMM (CK-3), CK-MB (CK-2), and CK-BB (CK-1). The CK-MB isoenzyme, accounts for 5 to 50% of total CK activity in myocardium. In skeletal muscle, by contrast, it normally accounts for just 1% or less, with CK-MM being the dominant form, though the percentage can be as high as 10% in conditions reflecting skeletal muscle injury and regeneration (e.g. severe exercise, muscular dystrophy, polymyositis). The CK-MB isoenzyme exists as 2 isoforms: CK-MB1 and CK-MB2. Laboratory determination of CK-MB actually represents the simple sum of the isoforms CK-MB1 and CK-MB2. CK-MB2 is the tissue form and initially is released from the myocardium after MI. It is converted peripherally in serum to the CK-MB1 isoform rapidly after symptom onset. Normally, the tissue CK-MB1 isoform predominates; thus, the CK-MB2/CK-MB1 ratio is typically less than 1. A result is positive if the CK-MB2 is elevated and the ratio is greater than 1.7. Serial measurement of biochemical markers is now accepted universally as an important determinant in ruling in or ruling out acute myocardial infarction. CK-MB is one of the most important myocardial markers (in spite of not being altogether cardiac-specific), with well-established roles in confirming acute myocardial infarction (AMI) and in monitoring reperfusion during thrombolytic therapy following MI.

CK-MB ELISA Test Principle:
In this test CK-MB calibrator, patient specimen or control is first added to a streptavidin coated well. Biotinylated monoclonal and enzyme labeled antibodies directed against distinct and different epitopes of CK-MB are added and the reactants mixed. Reaction between the various CK-MB antibodies and native CK-MB forms a sandwich complex that binds with the streptavidin coated to the well. After the completion of the required incubation period, the enzyme-CK-MB antibody bound conjugate is separated from the unbound enzyme-CK-MB conjugate by aspiration or decantation. The activity of the enzyme present on the surface of the well is quantitated by reaction with a suitable substrate to produce color. The essential reagents required for an immunoenzymometric assay include high affinity and specificity antibodies (enzyme conjugated and immobilized), with different and distinct epitope recognition, in excess and native antigen.

Country:	Hong Kong
Model No:	Cardiac Markers ELISA Kits
FOB Price:	(Negotiable) Get Latest Price
Place of Origin:	-
Price for Minimum Order:	-
Minimum Order Quantity:	-
Packaging Detail:	-
Delivery Time:	-
Supplying Ability:	-
Payment Type:	-
Product Group :	ELISA Tests