Dengue Combo NS1 Ag and IgG/IgM Ab Rapid Test kit

Product Description

The Dengue Combo NS1 Ag and IgG/IgM Ab  Rapid Test is a qualitative, membrane based immunoassay for the detection of NS1 antigen and IgG/IgM antibodies in human Serum/Plasma. Test consists two devices one device for detection of Dengue NS1 antigen and second device for the differential detection of  Dengue IgG/IgM antibodies. Dengue NS1 antigen device contains two lines;  C  (Control Line)  &  T (Test line). The test membrane is pre-coated with a NS1 specific antibody on the Test line region and utilizes a separate control to assure assay flow and performance. During testing, the test sample is added directly to the sample region and the 2 drops of  buffer is added  into the buffer well. The sample interact with NS*-specific monoclonal antibodies conjugated to gold nano particles. The solution migrates upward on the membrane (via capillary action) to react with the anti-NS1 antibody on the membrane. If  NS1 antigen is present, a purple/pink line will appear at the test line. The purple/pink line at the control region should always appear if the assay is performed correctly. The Dengue IgG/IgM Ab test consists of  two components, an IgG component and an IgM component. Dengue IgG/IgM Ab test device contains three lines, C (Control line) IgG and IgM (Tests Lines) IgM Test line is pre coated with anti-human IgG and IgM Ab Test line is pre coated with anti-human IgG. When a sample and buffer added to the device the mixture migrates upward on the membrane chromatographically by capillary action and reacts with anti-human IgG or IgM antibodies  coated on the membrane respectively, colloidal gold complex containing dengue antigens is captured by the anti- dengue IgG or IgM Ab on respective   T lines. If the specimen does not contain dengue antibodies no colored line will appear in either of the test line regions, indicating a negative result. To serve as a procedural control, a colored line will always appear in  the control line region, indicating that the proper volume of specimen has been added and membrane wicking has occurred.