FOB Price
Get Latest Price( Negotiable )
|1 Unit Minimum Order
Country:
USA
Model No:
E-EL-0026
FOB Price:
( Negotiable )Get Latest Price
Place of Origin:
USA
Price for Minimum Order:
-
Minimum Order Quantity:
1 Unit
Packaging Detail:
1KIT
Delivery Time:
within7~10 days
Supplying Ability:
-
Payment Type:
T/T
Product Group :
Contact Person Ms. Emily
14780 Memorial Drive, Suite 216., Houston, Texas
Catalog
number:Â E-EL****6
Size:Â *6T
Reactivity:Â Universal
Detection
Range:Â 1.*6~**0
μg/mL
Sensitivity:Â 0.*4
μg/mL
Lead
time:Â 7~*0daysÂ
Intended use
This ELISA kit can be applied to the in vitro quantitative
determination of GSH concentrations in serum, plasma and
other biological fluids.
Test principle
This ELISA kit uses Competitive-ELISA as the method. The microtiter
plate provided in this kit has been pre-coated with GSH. During the
reaction, GSH in the sample or standard competes with a fixed
amount of GSH on the solid phase supporter for sites on the
Biotinylated Detection Ab specific to GSH. Excess conjugate
and unbound sample or standard are washed from the plate, and
Avidin conjugated to Horseradish Peroxidase (HRP) are added to each
microplate well and incubated. Then a TMB substrate solution is
added to each well. The enzyme-substrate reaction is terminated by
adding Stop Solution and the color change is measured
spectrophotometrically at a wavelength of **0 nm ± 2 nm. The
concentration of GSH in the samples is then determined by
comparing the OD of the samples to the standard curve.
Specificity
This kit recognizes some recombinant and natural GSH. No
significant cross-reactivity or interference was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples
with low, middle and high level GSH were tested *0 times on one
plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples
with low, middle and high level GSH were tested on 3 different
plates, *0 replicates in each plate.
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 8 wells ×*2 strips | **0℃, 6 months |
Reference Standard | 2 vials | |
Concentrated Biotinylated Detection Ab (**0×) | 1 vial, **0 μL | |
Concentrated HRP Conjugate (**0×) | 1 vial, **0 μL | **0℃(shading light), 6 months |
Reference Standard & Sample Diluent | 1 vial, *0 mL | 4℃, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, *0 mL | |
HRP Conjugate Diluent | 1 vial, *0 mL | |
Concentrated Wash Buffer (*5×) | 1 vial, *0 mL | |
Substrate Reagent | 1 vial, *0 mL | 4℃(shading light) |
Stop Solution | 1 vial, *0 mL | 4℃ |
Plate Sealer | 5 pieces | Â |
Product Description | 1 copy | |
Certificate of Analysis | 1 copy |
Country: | USA |
Model No: | E-EL-0026 |
FOB Price: | ( Negotiable ) Get Latest Price |
Place of Origin: | USA |
Price for Minimum Order: | - |
Minimum Order Quantity: | 1 Unit |
Packaging Detail: | 1KIT |
Delivery Time: | within7~10 days |
Supplying Ability: | - |
Payment Type: | T/T |
Product Group : | ELISA Kits |