hav igm elisa diagnostic test kit

Product Description

COMPONENTS                                                                                      
 

1. Microplate                                                                                             *6 wells
2. Negative Control                                                                                 1 mL×1
3. Positive Control                                                                                   1 mL×1
4. Conjugate                                                                                              6 mL×1
5. Washing Buffer (*0×)                                                                         *0 mL×1
6. Substrate Solution A                                                                           6 mL×1
7. Substrate Solution B                                                                          6 mL×1
8. Stop Solution                                                                                        6 mL×1
9. Plate Cover                                                                                           3 pieces
10. Insert                                                                                                      1 copy

1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer  (*0×)with *9 volumes of distilled water, mix well.
2. Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control. After dispensing *0μL of sample or negative control or positive control to the respective wells. Gently vibrating the plate.
3. Incubate: Cover the Microplate with plate cover and incubate the Microplate in a thermostat-controlled water-bath or microplate incubator at *7? for *0 minutes.
4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted washing buffer ( *0~*0 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.
5. Add Conjugate: Add *0μL of conjugate to each well (except for the blank well).
6. Incubate: Cover the Microplate and incubate the plate at *7? for *0 minutes.
7. Wash the Plate: Repeat the wash procedure as in step 4.
8. Add Substrate: Add *0μL of Substrate Solution A and *0μL of Substrate Solution B to each well, mix well. Cover and incubate at *7? for *0 minutes.
9. Stop reaction: Add *0μL Stop Solution to each well, mix well.
10. Read the absorbance at **0 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from **0nm to **0nm.