Human papillomavirus IgM, HPV-IgM ELISA Kit

Product Description

Intended use This immunoassay kit allows for the in vitro quantitative determination of human papillomavirus IgM, HPV-IgM concentrations in cell culture supernates, serum, plasma and other biological fluids. Introduction Human papillomavirus (HPV) infection of the cervix uteri is associated with carcinoma of the cervix uteri. The human papillomaviruses are immunologically related to the bovine papillomaviruses: antisera reactive with both groups of viruses can readily be prepared. Papillomavirus capsid antigens have been demonstrated in pre-cancerous lesions and condylomatous tissue using such group-specific antisera prepared against viral capsids. Patients with genital warts, cervical intraepithelial neoplasia (CIN) and carcinoma of the cervix uteri have been reported to have higher serum IgG antibody levels to the group-specific capsid antigen compared to control groups. Test principle The microtiter plate provided in this kit has been pre-coated with antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antigen and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HPV-IgM, biotin-conjugated antigen and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of **0 nm � 2 nm. The concentration of HPV-IgM in the samples is then determined by comparing the O.D. of the samples to the standard curve.