R2-M Flow-through System

Product Description

Product Description

The R*-M “Flow through” hybridization device is a state-of-the-art liquid handling and temperature regulating device for membrane-based affinity capturing chemical processes such as DNA hybridization and protein immunoblot. The “Flow-through” hybridization technology overcomes the limitations caused by diffusion of reagents in other membrane-based systems. Using the highly efficient “Flow-through” hybridization platform reduces the time for DNA hybridization reactions from hours to minutes.

The “Flow-through” hybridization device eliminates the need for multiple water baths, shaking and liquid handling steps required with standard DNA hybridization and protein immunoblot applications. The closed waste disposal system contains the waste solutions avoiding possible contamination of the bench top.

The operation of the R*-M device is simple and convenient – just set the temperature and add the reagent solutions and let the device do the rest. The R*-M device can be operated manually or by user-defined programs.

Principle of the patented "Flow-through" Hybridization Technology

"Flow-through" Hybridization Technology provides the platform for rapid analysis of any analyte in the solution-Porous-Matrix System. This is exemplified by the DNA hybridization: It is characterized by active approach to facilitate the annealing reaction between the sample molecules (targets) and the capturing molecules (probes), which occur on three-dimensional porous matrixes rather than planar surfaces.

The annealing of two complementary DNA single strands is a very fast kinetic process when the two strands collide. However in the two-phase conventional hybridization system, the target molecules must move toward the probes fixed in the solid matrix during hybridization, Hence this diffusion through solid matrix - a slow process - is the rate-limiting step and therefore the incubation step usually takes hours or even overnight in order to achieve optimal results.

The "Flow-through" process overcomes this diffusion limitation during the annealing process and all subsequent steps as required for signal generation. By directing the targets toward the probes to form duplexes, the hybridization is changed from the passive and random diffusion to active channeling process allowing the recombination reactions to complete in seconds. The washing of non-specific and inbounded targets will be removed similarly. Theoretically, the "Flow-through" method will produce the optimal signal and signal-to-noise rations rapidly for any affinity capturing chemical processes: i.e. DNA-DNA; DNA-protein; Antibody-antigens; Ligand-receptor and other molecular interactions.

Added Values of "Flow-through" Process
-Ease of operation 
-Shorten hybridization and other processes duration to minutes 
-Simple solid support: Membrane i.e. Nylon membrane
-Less reagent consumption
-Open architectural platform
-Multiply analytes detection
-Adoptable to varies signal detection methodologies

Testing Kit Available on this platform

1. GenoFlow HPV Array Test Kit

2. GenoFlow HPV High Risk Screening Test Kit

3. GenoFlow DR-MTB Array Test Kit