Human C5a Elisa kit

Product Description

Elabscience Biotechnology Co., Ltd.
Package: *6T/kit
 
Intended use
This ELISA kit applies to the in vitro quantitative determination of Human C5a concentrations in serum, plasma and other biological fluids.
 
Detection Range
*8.*3 to ***0pg/mL.
 
Sensitivity
The minimum detectable dose of Human C5a is *6.*8pg/mL
 
Specificity
This kit recognizes natural and recombinant Human C5a. No significant cross reactivity or interference between Human C5a and analogues was observed.
 
Repeatability  
Coefficient of variation were  
Test principle
This ELISA kit uses Sandwich ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to C5a. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for C5a and Avidin Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C5a, biotinylated detection antibody and Avidin HRP conjugate will appear blue in color. The enzyme substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of **0 nm. The OD value is proportional to the concentration of C5a. You can calculate the concentration of C5a in the samples by comparing the O.D. of the samples to the standard curve.