Toxoplasma gondii antibody ELISA kit for Pet

Description

 Toxoplasma gondii antibody ELISA kitfor Pet
CodeSpec.Item1*6 wellsStop solution26 mlPositive control3**0 mlNegative control4*5 mlAdhesive Foil5*5 mlInstruction sheet6**0 mlSerum diluent plate1 piece  
3.Materialrequired notprovided
1)Microplate Reader with **0nm and **0nm
2) *7℃thermostatic device
3)Micropipettes, adjustable.
 
4. Samplerequirement
1) This kit is used for onlydog, cat, rabbit and moneyetc. serum and plasma
2) To get the best test result, avoid using sample with severe hemolysis, precipitate, contaminated by bacteria or protein suspension.
3)The serum sample store at **8℃ for 3 days, if for long term, it should be kept at **0℃or lower, avoid repeated freezing and thawing.
 
5.Test procedure
1) Washing Solution preparation: Dilute *0X Washing solutionwith distilled water or deionized water at 1:*0 (for example: take **0ml *0X Washing solution, add **0mldistilled water or deionized water, mix),mix it evenly to get washing solution.
2) Sample diluent and adding sample: Dilute serum with Sample diluent at 1:**0,Add**0μldiluted serum sample intoall samplewells; If using Serum diluent plate, the dilution method is like following:
Dilute at 1:**0: Firstly add *0ul sample diluent into the coated sample wells. On Serum diluent plate, add **5ul Sample diluent, then add 5ul serum, usePipette to mix it evenly, then take *0ul
 Intocoated sample wells for react. (Note: Serum diluent plate is disposable, can not repeat use.)
3)Add controls: Positive control and negative control do not need dilute, adddirectly. Set 2 blank controls, only add sample diluent,**0ul/well. Set 2 wells for negative control, only add **0ul negative control; 2 wells for positive control, only add **0ul positive control. Cover andIncubate in dark at *7 ℃ for30 minutes.
4) Washing plate: Discard the liquid of the well, add washingsolutioninto each wellfully(**0ul/well if using washing machine) for 3 times,incubate for1 min each time, at last time flap to dry with the absorbent paper.
5)AddingEnzymeconjugate: Add *0ul EnzymeConjugate into all wells (except blank well) andincubate at *7℃ in dark for30 minutes. Discard the contents of the wells and wash3 times as described in step 3.
6)Adding substrates: according to quantity needed, Add equal volume of Substrate A and Substrate B, mix evenly.Add **0ul into all well, incubate at *7℃ in dark for *0 minutes.
7) Add *0ul Stop solution into all wells to stop reaction. Set zero at blank control, read the OD value at ELISA reader **0nm (**0nm as a reference).