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Sell Avian Marek's disease virus antibody ELISA kit

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180 ~ 240 / Box

Minimum Order

Place of Origin:

Price for Minimum Order:

Minimum Order Quantity:

1 Box

Packaging Detail:

By foam box with ice to maintain cool storage

Delivery Time:

By FedEx in 3 days after confirming payment

Supplying Ability:

1000 Box per Day

Payment Type:

T/T, Western Union, Money Gram

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Shenzhen Lvshiyuan Biotechnology Co.Ltd

China

Free Member

Contact Person Bella

Shenzhen, Guangdong

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Description

Avian Marek\'s disease virus antibody ELISA kit
Catalog No. LSY***0*6
1. Usage
This kit is used to detectAvian Marek\'sdisease virus antibody in chicken serum, to assess antibody condition byAvian Marek\'s disease vaccine in chicken farm and assist diagnosis ofserological infected chicken.
2. Principle
The Avian Marek\'s disease antibody ELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (**0 nm).
3. Reagents

MD antigen coated microtiter strips 1 piece (*6 wells)
Enzyme Conjugate 1 bottle (*1mL/bottle)
10× Concentrated washing solution………………………………1 bottle (*0mL/bottle)
Substrate A /B solution 1 tube each (6 mL/tube)
Sample dilution………………………………………………….…. 1 bottle (*0mL/bottle)
Stopping solution 1 tube (6 mL/tube)
Negative control 1 tube (**0μL /tube)
Positivecontrol 1 tube (**0μL /tube)
Serum dilution plate……………………………………………………..…..1 piece
Adhesive film……………………………………………………………….2 pieces
Instruction……………………………………………………………………1 piece

4.Materials required but not provided
1)Micropipettors and disposable tips: 0.5μL~*0μL、*0μL~**0μL、**0μL~***0μL
2) *7℃Incubator
3) Measuringcylinder: **0 ml
4) *6 wells microplate reader
5) Distilled water or deionied water
6)Bottle or microplate washing machine
5. Sample preparation
Take animal whole blood, make serum according to regular methods, the serum should be clear, have no hemolysis.
6. Preparation of washing buffer
Return washing solution to room temperature before use, if there is salty crystals, shake to make the crystalsdissolve, then use distilled water or deionied water to dilute it at *0 times. The diluted washing solution can store for 1 week at 4℃.
7. Sample dilution
At serum dilution plate, dilute serum at 1:*9 with sample dilution (for example: **5μL sample dilution + 5μL serum)
Notice:Negative control and Positive control do not need dilute. Exchange tip after taking sample every time, record the situation of the sample on plate accurately. Shake the sample evenly before adding it.
8. Notes
1)All reagents should be adjusted to the room temperatureand shake evenlybefore using,store at **8℃ after using
2)Do not exchange the reagents from the kits of different lot numbers to use. Avoid reagent pollution when using.
3) Substrate and stop solution may have excitant to skin and eyes, pay attention when using.
4) Do not expose TMB (Substrate B) to light and avoid it contact with antioxidants.
5) The wells should avoid damp or touching water after unsealing (Put the un-using microplate back to bag with dehydrator in 2~8℃ soon )
6) Deal all waste reasonable before dumping to avoid pollution.
7) Strictly adhere to instruction to get best result. All procedure includingpipetting, timing and washing etc. must be accurate.
8) Serum dilution plate is disposable, do not use for second time; the MAX volume of it is **0μL/well.
9. ELISA procedure
1) Take pre-coated microplate (Can unseal for several time use as per sample quantity), add **0μL diluted serum to a well, meanwhile set 1 wells forNegative control, Positivecontrol and blank control wells separately. Add **0μL Negative/Positivecontrol to its wells, only add **0μL sample dilution in the blank control wells.Shake softly (do not spill),incubate at *7℃ for *0 min.
2)Pour the liquid out of the wells, add*50 μL diluted washing solution to each well, static for1 min, pour out. Repeat3 times, then pat to dry on absorbent paper.
3)Add **0 μL Enzyme Conjugate solutionto each well, andincubate at *7℃ for *0 min.
4)Repeatthestep2(washing). Rememberpat to dry on absorbent paper at last.
5)Add *0 μL substrate A, then substrateB (*0 μL) to each well, mix properly,react for *0 min at *7℃ in dark.

Add *0 μL stopping solution in eachwell, and measure the result within *0 min.

*0. Results
Set zero for the blank well, and test theA**0nm(**0 nm as a reference)value on the microplate-reader. The conditions for the test to be tenable are that the positive control wells averageA*50nm value isgreater than or equal to 0.6, and the negative control wells averageA*50nm value isless than0.*5. If the test is invalid, the operation is in doubt, retest and observe all the reagents carefully.
If the samples A**0 value is greater than 0.2+absorbance of negative control average value, it is judged to be positive; and if less than 0.2,negative. Ifabsorbance of negative control mean is less than 0.*5, calculate as 0.*5
Specifications: *6 wells/kit.
Expiry date:*2months.
Storage: Storing at**8℃, in the dark.

Sell Avian Marek's disease virus antibody ELISA kit

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Sell Avian Marek's disease virus antibody ELISA kit

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