Description
Taq DNA Polymerase (Buffer contains magnesium ions) Product number:
T***2
Storage conditions: **0 ℃. Concentration: 5U/µl.
Product introduction
Taq DNA Polymerase is isolated and purified from Escherichia coli
cloned with Thermu aquaticus DNA Polymerase gene after induction,
and its molecular weight is *4 KD. Taq DNA Polymerase has 5′*3′ DNA
polymerase activity and 5′*3′ exonuclease activity, but no 3′*5′
exonuclease activity. In the PCR reaction, the extension rate of
Taq DNA Polymerase is **2 kb/min, and the product has A at the 3′
end, which can be directly used for T/A vector cloning.
Product composition
Taq DNA Polymerase **0U
*0xTaq Buffer+（with MgCl2） 1ml
Activity unit
1 unit (U) of Taq DNA Polymerase activity is defined as the amount
of enzyme required to incorporate *0 nmol of deoxynucleotides into
acid-insoluble substances using activated salmon sperm DNA as a
template primer at *4C within *0 minutes.
Quality control
The purity detected by SDS-PAGE is greater than *9%, and no
exogenous nuclease activity is detected; the PCR method detects no
host residual DNA, and can effectively amplify single-copy genes in
the human genome; there is no obvious change in activity after
being stored at room temperature for one week.
Enzyme storage buffer
*0mMTris-HCl (pH8.0), 0.1 mM EDTA, 1mM DTT, **0 mM KC1,
Stabilizers, *0% glycerol.
*0XTaq Buffer (containing Mg2):
**0 mM Tris-HCl (pH 8.4), **0 mM KC1, **0 mM (NH)z SO, *5 mM MgCl2,
other ingredients.
★ *0X Taq Buffer is divided into two types: containing Mg°2+ and
not containing Mg2+, which can be selected.
★ Buffer without Mg2+ is also equipped with *5 mM MgCl2.
★ If not specified, Buffer containing Mg2+ is usually provided.
Scope of application
Generally used for PCR amplification of DNA fragments, DNA
labeling, primer extension, sequence determination, blunt end A
addition, etc. The product can be directly used for T/A vector
cloning.
Recommended PCR conditions: (Take *0 μl reaction system as an
example)
Template <0.5 μg
Forward Primer (*0 μ M) 1 μl
Reverse Primer (*0 μ M) 1μ1
*0XBuffer* (with mgcl2) 5 μl
dNTP Mixture (2.5mM each) 4 μl
Taq DNA pol ymerase (5U/μ 1) 0.5~1 μ 1
dH*0 up to *0μ1
PCR reaction cycle settings:
1.*4°C:**5 min
2.*4C:*0 sec
3.****0°C:*0 sec
4.*2*C:1 min/**2 kb
5.*2C:***0 mins
(2~5 : *0cycles)
Precautions
For your safety and health, please wear a lab coat and disposable
gloves when operating.
This product is for experimental research only.
