FOB Price
Get Latest Price300 ~ 500 / Box ( Negotiable )
|1 Box Minimum Order
Country:
China
Model No:
CK-E90222
FOB Price:
300 ~ 500 / Box ( Negotiable )Get Latest Price
Place of Origin:
china
Price for Minimum Order:
300 per Box
Minimum Order Quantity:
1 Box
Packaging Detail:
NEUTRO-PACKING
Delivery Time:
3-5days
Supplying Ability:
1000 Box per Week
Payment Type:
T/T, Western Union, Money Gram
Product Group :
Contact Person Ms. Ada
Room 15A12, Building 1, Blue Ocean International Times Mansion, No.39 Yile road, Hangzhou, Zhejiang
Human epidermal growth factor
receptor(EGFR)ELISA Kit
Please carefully read this instruction before using.
This ELISA kit is based on the principle of double-antibody
sandwich technique to detect Human epidermal growth factor
receptor(EGFR)ELISA Kit. Be used only for research purposes, not be
used for medical diagnosis.
【Intended Use】
This
kit is used to assay the epidermal growth factor receptor(EGFR) in
the sample of Human ’s serum, blood plasma, and other related
tissue Liquid.
【Test principle】
The kit uses a double-antibody sandwich
enzyme-linked immunosorbent assay (ELISA) to assay the level of
Human epidermal growth factor receptor(EGFR) in samples. Add
epidermal growth factor receptor(EGFR) to monoclonal antibody
Enzyme well which is pre-coated with Human Krebs yon epidermal
growth factor receptor(EGFR)antibody, incubation; then, add Krebs
yon epidermal growth factor receptor(EGFR)antibodies labeled with
biotin, and combined with Streptavidin-HRP to form immune complex;
then carry out incubation and washing again to remove the
uncombined enzyme. Then add Chromogen Solution A, B, the color of
the liquid changes into the blue, And at the effect of acid, the
color finally becomes yellow. The chroma of color and the
concentration of the Human Substance Krebs yon epidermal growth
factor receptor(EGFR)of sample were positively correlated.
Materials supplied in the Test Kit
1 |
Standard |
0.5ml |
7 |
Chromogen Solution A |
6ml |
2 |
Standard diluent |
3ml |
8 |
Chromogen Solution B |
6ml |
3 |
Microelisa Stripplate |
*2w×8s |
9 |
Stop Solution |
6ml |
4 |
Str- HRP-Conjugate Reagent |
6ml |
*0 |
Instruction |
1 |
5 |
*0×wash solution |
*0ml |
*1 |
Closure plate membrane |
2 |
6 |
Biotin-EGFR-Ab |
1ml |
*2 |
Sealed bags |
1 |
Materials
required but not supplied
1. *7 °C incubator
2. Standard Enzyme reader
3. Precision pipettes and Disposable pipette
tips
4. Distilled water
5. Disposable tubes for sample
dilution
6. Absorbent paper
Summary
Procedure
Preparing reagents, samples and standards
Add prepared samples and standards, antibodies labeled with enzyme, reacting 60 minutes at *7 °C
Plate washed five times, adding Chromogen solution A, B, reacting 10 minutes at *7 °C
Add stop solution
Measure the OD value within 10min
Package size:*6T
per box.
Validity&Storage:Six
months(**8°C)orTwelvemonths(**0°C[see
label on the outer box for the specific date].
Country: | China |
Model No: | CK-E90222 |
FOB Price: | 300 ~ 500 / Box ( Negotiable ) Get Latest Price |
Place of Origin: | china |
Price for Minimum Order: | 300 per Box |
Minimum Order Quantity: | 1 Box |
Packaging Detail: | NEUTRO-PACKING |
Delivery Time: | 3-5days |
Supplying Ability: | 1000 Box per Week |
Payment Type: | T/T, Western Union, Money Gram |
Product Group : | elisa kit |