Human platelet-activating factor acetylhydrolase, PAFA ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human PAFA concentrations in plasma.

 

Introduction

Phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma), also known as PLA2G7, is a human gene.

The PLA2G7 gene encodes platelet-activating factor (PAF) acetylhydrolase (EC 3.1.1.*7), a secreted enzyme that catalyzes the degradation of PAF to inactive products by hydrolysis of the acetyl group at the sn*2 position, producing the biologically inactive products LYSO-PAF and acetate.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PAFA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PAFA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PAFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of **0 nm ± 2 nm. The concentration of PAFA in the samples is then determined by comparing the O.D. of the samples to the standard curve.