Human soluble Vascular cell adhesion molecule 1,sVCAM-1 ELISA Kit

Product Description

Method：sandwich

Detection range：0.1ng/ml→*0ng/ml

Sensitivity：0.*5ng/ml

Assay length：**5Hours

Detection Wavelengt：**0nm

Research Area：Immunology

Price：inquiry

Quality guarantee period：for *2 months

Introduction

INTENDED USE

The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

STORAGE

For unopened kit: All the reagents should be kept according to the labels on vials, Washing concentrate (*0X) and the Stop Solution should be stored at 4℃ upon receipt while the others should be at **0℃

PRECISION

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested *0 times on one plate,respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX**0

Intra-Assay: CV<8%  

Inter-Assay: CV<*0%

ASSAY PROCEDURE SUMMARY 

1. Prepare all reagents, samples and standards; 

2. Add prepared samples, standards and ELISA solutions. Let them react for *0 minutes at *7 ℃.

3. Wash the plate five times. Add Chromogen solution A and B. Incubate for *0 minutes at *7 ℃; for color development.

4. Add stop solution

5. Read the OD value within *0minutes. 

6. Calculate