Pfu DNA Polymerase

Product Description

Description

Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase. Its molecular weight is *0 kDa. It can amplify DNA target up to 2 kb(simple template). The elongation velocity is 0.2~0.4kb/min(*0~*5°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

 

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of *0 nmole of dNTPs into an acid-insoluble form in *0 minutes at *0°C using hering sperm DNA as substrate.

 

Storage Buffer

*0mM TrisCl ( pH8.0), **0mM KCl, 3mM MgCl2 1mM DTT,0.1% NP**0 ,0.1% Tween*0, 0.2mg/ml BSA, *0% (v/v) glycerol

 

*0X Pfu Buffer with MgSO4 

**0mM Tris-HCl(pH8.8,*5°C), **0mM KCl, **0mM (NH4)2SO4,  *0mM MgSO4, 1.0% Triton®X***0, 1mg/ml BSA

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Applications

• High-fidelity PCR and primer-extension reactions.

• High fidelity PCR for cloning into blunt-ended vectors.

• Site-directed mutagenesis.