Recombinant Human Keratinocyte Growth Factor-2(KGF-2)

Product Description

Recombinant Human Keratinocyte Growth Factor*2 Description Human Keratinocyte growth factor*2 (KGF*2 or FGF**0) is a novel human protein that stimulates the repair of injured mucosal tissues. KGF*2 directly promotes wound-healing by stimulating the proliferation, migration and differentiation of epithelial cells, and has direct chemotactic effects on tissue remodeling. KGF*2 also appears to result in less scarring. It was found to induce healing in skin and mucosal cells that had been damaged, but had no effect on healthy cells. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a concentrated (1mg/ml) solution in water containing *0mM Sodium Phosphate buffer, pH 7.4 , *0 mM NaCl. Solubility ��It is recommended to reconstitute the lyophilized KGF *2 in sterile *8MΩ-cm �H2O not less than **0ug/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized KGF*2 although stable at room temperature for 3 weeks, should be stored desiccated below **8 C . Upon reconstitution KGF*2 should be stored at 4 C between **7 days and for future use below **8 C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. Purity Greater than *5.0% as determined by: (a) Analysis by RP-HPLC. (b) SEC-HPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained xxxxx Amino-Acid Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Leu-Gly-Asp. Dimers and aggregates Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Biological Activity Recombinant human KGF*2 is fully biologically active when compared to standards. The specific activity is about 1.0 x *0 6 Units/mg.