ROC-F-OR003K Melamine ELISA Test Kit

Product Description

ROC-F-OR**3K Melamine ELISA Test Kit
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Melamine in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Melamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Melamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Melamine concentration in the sample. This value is compared to the standard curve and the Melamine concentration is subsequently obtained.
 
2. Technical specifications
Sensitivity:  0.5 ppb
Incubator temperature:  *5℃
Incubator time:  *0min～*5min
Detection limit:
Milk powder................................................................................... *0 ppb
Milk................................................................................................. 1 ppb
Feed.............................................................................................. *0 ppb
Cross-reaction rate:
Melamine......................................................................................... **0%
Cyanuric acid..................................................................................... *0%
Triazine.............................................................................................. <1%
Diamino atrazine................................................................................ <1%
Recovery rate:
Milk.............................................................................................. *0±*5%
Milk powder.................................................................................. *0±*5%
Feed............................................................................................ *0±*5%
 
3. Components

1. Micro-well strips: *2 strips with 8 removable wells each
2. 6× standard solution (1 mL each): 0 ppb,0.5 ppb, 1.5 ppb, 4.5 ppb, *3.5 ppb, *0.5 ppb
3. Enzyme conjugate (7 mL)............................................................... red cap
4. Antibody working solution (7 mL).................................................. blue cap
5. Substrate A solution (7 mL)......................................................... white cap
6. Substrate B solution (7 mL)......................................................... black cap
7. Stop solution (7 mL).................................................................. yellow cap
8. *0× concentrated washing buffer (*0 mL)..................................... white cap
9. 2× concentrated redissolving solution (*0 mL)..................... transparent cap