Trans2K Plus DNA Marker

Product Description

Cat. No.BM**1

Ordering information

Storage:at 4oC for six months; at **0oC for two years

Concentration:0.*7 mg/ml

Description

Trans2K® Plus DNA Marker is composed of eight linear double-stranded DNA bands. This ready-to-use product is premixed with 1 × DNA loading buffer. Based on experiment requirement, 5 μl marker can be directly loaded for gel electrophoresis. It provides simple operation and clear image. For easy reference on agarose gels, the **0 bp band (**0 ng/5 μl) is two times brighter than the other bands (*0 ng/5 μl) in the ladder.

Advantages

• Clear bands, easy determination

• Simultaneous determination of the size of enzyme-digested vector and inserted fragments

Composition

**0 bp, **0 bp, **0 bp, **0 bp (**0 ng/5 μl, the double intensity band), ***0 bp, ***0 bp, ***0 bp, ***0bp

Storage Buffer

*0 mM Tris-HCl (pH 8.4), *0 mM EDTA, 0.*2% bromophenol blue, 5% glycerol

Instructions for Use

• Load Trans2K® Plus DNA Marker on the lane on a 1.5% agarose gel (load 1 μl marker for 1 mm lane, increase the loading volume for wider lane ).

• Suggested electrophoresis conditions: 1.5% agarose gel, voltage ***0 v/cm.

• Stained with EB or GelStain (GS**1), visualize the bands under exposure to ultraviolet light.

Notes

• Completely thaw and mix well before use.

• Please change the electrophoresis buffer in time and use freshly-prepared gel to avoid adverse on the result.

CITATIONS

Yu Q, et al. ***3. Functional Analyse of GLUT1 and GLUT*2 in Glucose Uptake in Goat Mammary Gland Epithelial Cells. 8(5):e****3. PLoS One. IF=3.*3. PMID: *******4.

Long D, et al. ***3. In Vivo Site-Specific Integration of Transgene in Silkworm via PhiC*1 Integrase-Mediated Cassette Exchange. *3(*1):*******8. Insect Biochem Mol Biol. IF=3.**4. PMID: *******0.

Dai J, et al. ***3. A novel DNA vaccine expressing the Ag*5A-HA2 fusion protein provides protection against influenza A virus and Staphylococcus aureus. *0:*0. Virol J. IF=2.**2. PMID: *******0. 

Xu X. et al. ***2.Hypoxia induces downregulation of soluble guanylyl cyclase β1 by miR**4c*5p. **3(**7):****8. IF= 6.**1.  PMID: *******7.

Wu CX. et al. ***0.The Zalpha domain of PKZ from Carassius auratus can bind to d(GC)(n) in negative supercoils. *8(**6):****8. IF= 3.**2.  PMID: *******4.

Guo Y. et al. ***2.Association between calcifying nanoparticles and placental calcification. 7:******6. IF= 3.*3.  PMID: *******1.

Long DP. et al. ***2.FLP recombinase-mediated site-specific recombination in silkworm, Bombyx mori. 7(6):e****0. IF= 4.**2.  PMID: *******5.

Dai J. et al. ***2.Molecular adjuvant Ag*5A enhances protection against influenza A virus in mice following DNA vaccination. 4(*2):******4. IF=1.5.  PMID: *******5