Aspergillus Fluorescence PCR Kit

Product Description

~Aspergillus Detection Kit Instruction (Fluorescence PCR Method)
Quantitative detection for Aspergillus DNA in human .
*0 Tests
(Fluorescence PCR Method)
ITEM NO. PCR***1

Overview
The Aspergillus Detection Kit is suitable for the quantitative detection of human blood and  deep infection in alveolar lavage aspergillus, can be used for auxiliary diagnosis and its  infection patient monitoring the efficacy of drug treatment of aspergillus fumigatus,  aspergillus flavus, aspergillus nidulans, aspergillus niger, aspergillus terreus, aspergillus  clavatus, aspergillus versicolor . Test result is only for clinical reference, cant be used as the basis of confirmed or excluded cases alone.

Assay Characteristics
Basic Parameters 
Item No. PCR***1
Method Fluorescence PCR Method
Sample type Blood, BALF
Specification *0 tests/kit
Detection time 2 hs
 1.0 x **2 gene  copy,
Linear range 1.0×**3～1.0×**7 gene copies
Inter assay variation ≤*5%
Detection objects Aspergillus
Stability The kit is stable for 4 weeks at 4 ℃ after opening, and the variability is no more than *5%.
Machine to operate on Fluorescence ration PCR Instrument

Principle of the Test
This kit use a pair of specific primers and a specificity of fluorescent probe, PCR reaction liquid, heat-resistant DNA polymerase, nucleotide monomers (dNTPs), detection deep infection aspergillus DNA by PCR amplification in vitro method.

Clinical Significance
The incidence of invasive aspergillus infection (IA) by both primary and opportunistic pathogens, has been steadily increasing among hematological malignancy and AIDS patients and the patients receiving aggressive chemotherapy for cancer, bone marrow and organ transplantation or advanced critical care. The difficulty in diagnosis of these infections are associated with significant mortality and morbidity. Since the traditional methods for the aspergillus infection detection such as blood culture and histopathological diagnosis are either less sensitive or not feasible in the critically ill patients, developemnt of untraditional method for the IFI diagnosis is of great importance.
Among the new diagnostic techniques is the assay for the aspergillus detection. Positive in the PCR test has been recognized as a very important microbiological evidence in the clinical diagnostic criteria for invasive aspergillus infection.

Samples Collection and Storage
1. Appllied specimen type: Human WB, BALF.
2. Blood specimen collection：take blood 1mL by disposable syringe，to the anticoagulant tube，enclosed inspection.
3. Alveolar lavage fluid collection：after collecting the alveolar lavage to enclosed inspection.
4. Sample  preservation  and transport：specimens can be used to test immediately, also can be saved in **0 ℃ for inspection pending. Specimen transport should be 0 ~ 8 ℃ low temperature.

Result Interpretation
1. If growth curve type is not in an s-shaped curve or Ct value > = *5，then determine the total content of the sample is less than the limit of detection.
2. If growth curve type in an s-shaped curve and Ct value 1.0×**7 gene copies. If you need accurate quantitative results，can detection after dilute the sample of extracted to linear range.